Application

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Signaling

This Anti-KChIP2 potassium channel subunit Antibody, clone 60/73 is validated for use in western blotting & IHC for the detection of KChIP2 potassium channel subunit.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected KChIP2 potassium channel subunit in human cerebral cortex tissue.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

KCHIP2, also known as Kv channel-interacting protein 2 (KChIP2), or A-type potassium channel modulatory protein 2, or Potassium channel-interacting protein 2, and encoded by the gene Kcnip2/Kchip2, is the regulatory subunit of the Kv4/D Shal-type voltage gated inactivating potassium channel. KCHIP2 modulates the activities of Kv4.2 and Kv4.3 potassium channels. KCHIP2 is localized to the cell membrane but is a peripheral membrane protein. KCHIP2 is expressed in heart, brain and lung. In brain, KCHIP2 is abundantly expressed in striatum, hippocampus, and olfactory bulb, but moderate to low expression is seen in cerebral cortex thalamus and hypothalamus. KCHIP2 has multiple isoforms in the different tissues. Isoform 1 is predominant in cerebral cortex, striatum and hippocampus, and all three isoforms are equally expressed in olfactory bulb, but in heart only isoform 3 predominates.

Immunogen

GST-tagged recombinant protein corresponding to rat KChIP2 potassium channel subunit.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected KChIP2 potassium channel subunit in 10 µg of mouse brain tissue lysate.

Specificity

This antibody does not cross react with rat KChIP1 and KChIP3 (Prof. J. Trimmer, University of California, Davis).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 35 kDa observed. Uncharacterized band(s) may appear in some lysates.